NovaFISH probe design
Product Pipeline -
Product Name：
NovaFISH
Product Type：
RNA in situ hybridization assay kit
Dye Label：
Our NovaFISH probes can completely be customized based on any gene sequence you choose.
This NovaFISH kit includes NovaFISH probes and other necessary consumables to get you started immediately.
How does NOVAFISH work?
NovaFISH is a novel single-molecule fluorescence in situ hybridization (smFISH) technique. NovaFISH utilizes our proprietary new Modality Enzymatic Convergent Assembly(nMECA) platform, allowing each probe to be simultaneously labeled with multiple colors. This innovative approach not only reduces costs, but also enhances staining efficiency. With the capability to mutliplex, NovaFISH probes allow for the simultaneous detection of the expression of 7 genes in a single hybridization process, and with more channels on the microscope, it can perform detection of 7-100 genes.
With an ever-increasing range of spectrally separable fluorophores, the diversity of NovaFISH expands exponentially. This means, that more probes labeled with different colors can be used simultaneously on the same sample, enabling the detection and differentiation of more target sequences or structures concurrently. This increased diversity provides more comprehensive and accurate information for the study of chromosomal structures and clinical diagnostics.
Multiple Signals - Providing Comprehensive, Accurate, and Reliable Information
The complexity and diversity of biological systems represent challenges in studying gene expression. The intricacy and redundancy cannot be ignored when investigating pathway-related genes or biomarkers. In such cases, the application of multiple biomarkers boosts research accuracy. NovaFISH and similar methods are very useful tools to accurately identify all genes of interest. They offer real expression patterns with spatial information, enhancing our understanding of individual gene functional mechanisms.
By utilizing multiple signals, we gain comprehensive insights into the functionality and regulation of biological systems. A single signal may not fully reveal the complexity of gene expression, as genes interact and regulate each other. However, employing multiple biomarkers allows simultaneous observation of different genes and their expression patterns in space and time. This facilitates a deeper understanding of gene interactions, regulatory networks, and cellular functions.
Multiple signals offer key validation aspects. Simultaneously detecting multiple markers in the same sample validates research results, eliminating potential interfering factors and ensuring research reliability and accuracy.
Our Advantages
Unique Enzymatic Labeling Probes
Compared to traditional fluorescently labeled probes, the advantage of enzymatically labeled probes is amplifed fluorescent signal, thus enhancing the detection sensitivity of the probes.
Multiplex Detection
NovaFISH enables multiplex detection, improving high-throughput analysis efficiency.
Multiplexing Capability
NovaFISH's multiplexing capability grows exponentially with more fluorescent dyes. As the dye options increase, it enables broader gene expression analysis by detecting multiple genes simultaneously.
High Resolution
NovaFISH utilizes enzymatically labeled probes to enhance detection sensitivity and resolution, enabling precise quantification and localization of gene expression at the single-molecule level.
Easy Operation
Compared to other cascade amplification techniques like RNA scope, NovaFISH is simpler and easier to operate in terms of sample preparation, reagent usage, time efficiency, equipment requirements, and technical difficulty.
NovaFISH的应用方向
Applications of NovaFish
Gene Expression Regulation
Gene Expression Regulation
NovaFISH detects and quantifies individual RNA or DNA molecules at the cellular or tissue level, offering insights into gene expression regulatory mechanisms like transcriptional dynamics, cellular heterogeneity, and variability. At the single-molecule level, it reveals individual variations and complexities within gene regulatory networks in cell populations.
Cell Differentiation and Development
Cell Differentiation and Development
This method tracks and analyzes gene expression patterns during cell differentiation and development. By localizing and quantifying target RNA or DNA molecules in cell lineages, it reveals transcriptional differences and dynamic changes between different cell types.
Disease Mechanisms and Diagnostics
Disease Mechanisms and Diagnostics
By detecting and localizing abnormal expression of pathologically relevant genes, NovaFISH reveals molecular changes linked to diseases. Moreover, it can be used for disease diagnosis and classification, such as the identification of cancer subtypes and screening for molecular markers.
Cell-Environment Interactions
Cell-Environment Interactions
By detecting the expression of specific genes inside cells and signaling molecules in the extracellular environment, it reveals cell responses to external stimuli and signaling mechanisms.
We provide high-quality NovaFISH probes tailored to meet your research and experimental requirements. Our NovaFISH probes are meticulously designed and optimized to deliver excellent specificity and sensitivity, ensuring accurate hybridization and detection results. We employ the latest technologies and stringent quality control standards to guarantee the reliability and consistency of the probes. As an example, the electrophoresis test results of the size of the NovaFISH Probe example (Figure 1). Meanwhile, the maximum excitation wavelength of the ATTO 488 fluorescent group is 504nm, as shown in Figure 2, from the full wavelength scan, our NovaFISH have a single absorption peak at 504nm.
We welcome you to order our NovaFISH probes and acquire high-quality research tools. Whether you require custom probe sequences, probes for specific regions, or combinations of probes for multiple loci, we can design and prepare them according to your precise requirements. Our team has extensive experience and expertise to provide you with customized solutions that will perfectly align with your specific research needs.
Please, feel free to contact us for more information about our NovaFISH probes. We look forward to collaborating with you and providing high-quality probes for your research.
