Welcome to share your valuable message with us through our website! To facilitate seamless communication and to ensure prompt responses, we ask you to provide a valid e-mail address and a phone number. We are eager to establish a fruitful cooperation with you and offer you services of the utmost quality.
Please, be assured that we strictly adhere to all pertinent privacy policies and legal obligations, protecting your personal information from disclosure, misuse, or unauthorized access.
Thank you for your message and for providing us with your contact information. We will contact you as soon as possible.
nMECA, or New Modality Enzymatic Convergent Assembly is our patented technology that can be summarized as follows: short sequences are chemically synthesized right until the efficiency of the chemical reaction starts decaying. At that moment, the synthesis is stopped and, instead, two sequences are assembled by a ligase. This biochemical process is notably more efficient, resulting in fewer side products and thus, enhanced purity. The higher purity has an immediate impact on the outcome of your experiment, for there will be fewer functional sequences competing for Cas9, thus, increasing the specificity of your experiment.
In other and fewer words, your total sequence is chemically synthesized in several short fragments, and these are then assembled, using a ligase, to obtain the final sequence. This approach also improves significantly the batch-to-batch identity of the sequences.
You can purchase this product directly from our official website. Please, visit our online store and select the desired product and quantity on the shopping page. Then, carefully follow the instructions provided to smoothly complete the purchasing process. You can also contact us via e-mail for purchasing.
Yes, we offer international shipping services. Please, kindly ensure that you provide the accurate delivery address during the checkout process. While selecting the shipping method, you can review the applicable shipping fees and estimated delivery time for international orders.
The delivery time is influenced by various factors, including product type, stock availability, order quantity, and logistic arrangements. We strive to process and arrange delivery as soon as possible upon receiving your order. The actual delivery time may vary depending on the specific product; however, on average, we dispatch the product within approximately 7 working days from the time of order.
The ideal microscope for your NovaFISH probes is a confocal microscope of an epifluorescence microscope. We recommend to have available an immersion objective of minimally 63x and a set up with the camera that allows you a resolution of, at least, 100 nm/pixel. These requirements will allow you to quantify more accurately the number of signals. However, if you do not need to quantify and a qualitative result is enough for your experiment, a simpler set-up should be possible.
No. What we provide is a pool of sequences, by default 32. The number of sequences in the pool can be optimized according to your experimental needs. We have had customers who requested as few as 6 sequences per pool, and customers for whom we designed 96 sequences per pool, for example, if the target sequence is long enough, but is known to have low abundance in the sample. Typically the sequences have lengths of 20 nt. Based on that, you can decide how much of the target you want to cover or adjust to what is possible, if you have a very short target.
By default, 3. However, we have proven that even 1 fluorophore per sequence is enough to visualize under optimal conditions. If you require high fluorescence, you can purchase our NovaFISH Plus Probe, where we can comfortably attach 9 fluorophore units to increase the signal intensity.
Lyophilized at -20℃, stable for years. Upon reconstitution in water and storage at -20℃, stable for up to one year. When diluted in NovaHyb and stored at -20℃, stable for up to two years.
Yes, our standard NovaFISH probes have 3 fluorophores per sequence (9 in the case of NovaFISH Plus Probes for FFPE) of the same type. However, upon request, it is possible to produce a Probe with, for example, 1x Atto488 and and 2x Atto565; or 2x Atto448 and 1x Atto565, etc. These probes are a very good option for FRET assays and multiplexing detection via combinatorial barcoding.
Even though our default fluorophores are Atto488, Atto565, and Atto647N, we can use any fluorophore in any point of the spectrum. This, combined with a state-of-the-art microscope with 7 channels, the combined signals in areas of co-localization will give unique color codes that can facilitate the simultaneous detection of up to 120 genes.
Additionally, unlike many FISH Probes, multiple NovaFISH Probes can be used in the same hybridization round and at 37℃, thus, ensuring sample integrity and maximizing utility.
Yes, the probes can be used in any cells or tissues of any (micro)organism, as long as the exact target sequence is known.
Some of our customers have used our NovaFISH probes for applications such as FACS, co-localization of transcription and translation (combined with immune cytochemistry), etc.
The fluorophores listed in our online shop are available for immediate production of NovaFISH Probes. However, our nMECA technology allows us to use any fluorophore (such as Alexa, FITC, fluorescein, etc.). In such specific cases, please, contact us at order@pixelbscioences.com
We have sgRNA available in two grades: desalted and HPLC. Both surpass the average purity available in the market. We guarantee a purity higher than 90% in those purified through HPLC.
We produce from very standard sgRNAs of 20 nt for the guide sequence, and a total length of about 100 nt, to sequences as long as 190 nt. Longer sequences can be possible but would require longer time to produce and purify. For such cases, please, contact us directly at order@pixelbscioences.com where we can provide personalized care to your inquiry.
Common modifications such as Phosphorothioate, 2'-O-methyl RNA, 2'-fluoro RNA, Biotin, GalNAC, DNA bases, various fluorescent and quenching group modifications can be added. In addition to these, there are other modifications available for selection. If you have specific modification requirements or would like to explore more options, please feel free to contact us.
The price of these modifications varies according to the number of them within the sequence and the total amount of the product (in nmol). For specific inquiries, please contact us at order@pixelbscioences.com
The amount of default we deliver is 1.5 nmol of sequence. However, we can produce up to 500 nmol (lower mg scale). These larger orders may take longer time produce and deliver. Please, contact us at order@pixelbiosciences.com for more specific information.
Yes, our nMECA technology allows us to approach the production simply as products of nucleotides. The function of these products relies on the design, the sequence, and the chemical modifications. Thus, fluorophores would be attached to sgRNAs as they are attached to our NovaFISH probes. Given the form the fluorophores are attached to the sgRNA sequence, there is no steric hindrance that would prevent the interaction with the target gene, thus, maintaining the high performance of our sgRNAs, as our own research shows.
The stability of sgRNAs (single-guide RNAs) in cell culture conditions can vary depending on several factors, including the specific sequence, chemical modifications, and cellular environment.
Unless specified otherwise, all of our sgRNAs are provided with double modifications (2'-O-methylation and phosphorothioate) at the three first and last nucleotides of the sequence. These modifications significantly enhance the stability of sgRNAs in cell culture conditions by increasing resistance to nucleases present in the cellular environment.
For desalted sgRNA:
-Sequence of the guide
We produce the sgRNAs with an 80nt SpCas9 scaffold, with phosphorothioate and 2'-O-methyl modifications present on the first three and last three bases of the sgRNA sequence.
For HPLC sgRNA:
-Sequence of the sgRNA
-Amount in nmol
-Desired chemical modifications, if you need any
-Fluorophores and number of units in the sequence, if you need any
Up to date, we can provide sequences up to 190 nt. Longer sequences may be possible, but it may require longer turnovers and additional development.
In case that is needed, please, contact us at order@pixelbiosciences.com for more specific and guided assistance.
After HPLC purification, additional to the reduced amounts of side products, consequence of our assembly technology nMECA, we guarantee purities of, at least, 90% in your pegRNAs.
The purification of pegRNA and other long nucleotide sequences becomes more difficult as the length increases. Given that pegRNAs are intrinsically very long very sequences, we strongly recommend using HPLC-purified pegRNAs only. However, it is technically possible under requests.
Our production facilities follow GMP standards and every pegRNA is purified and analysed with HPLC. Additionally, we conduct Mass Spectrometry (MS) analysis and PAGE analysis for every product to ensure quality and purity.
CRISPR FISH usually incorporates three fluorophore molecules for signal detection and visualization.
We had offered products labeled with Cy3 and Cy5 fluorophores.You can contact us for various other types of ATTO fluorescent labels.
Our kit includes sgRNA, NovaFISH probes, along with their associated NovaHyb and NovaWash. In addition, you'll need to prepare the following reagents: Cas9 nickase H840A、RNase inhibitor、Binding or Blocking buffer、T7 EXO.
CRISPR-ExoFISH eliminates the need for heat denaturation, requiring only a compact region below 5 kb for DNA FISH probes to bind and generate specific signals.
