If you have been working with us for a couple of years, you probably already noticed that our single-molecule FISH Probes changed names and are not called HuluFISH Probes anymore, but NovaFISH Probes. However, not only was it the name that changed, but also did the structure.

In the field of genetic research, DNA FISH has long been a reliable technique for elucidate chromosomal mysteries, bringing the spotlight on chromatin organization and diagnosing clinical abnormalities. However, its dependence on heat denaturation and the need for lengthy target regions have represented significant obstacles, particularly when dissecting fine chromatin structures below 10 kilobases (kb), such as those introduced by exogenous genes during cell and gene therapy.

At PixelBiosciences, we are deeply concerned about the COVID-19 pandemic. Therefore we are developing a new research tool for COVID-19 with our NovaFISH technology. NovaFISH technology is customizable for any gene in any organism, allowing scientists to detect their genes of interest at the single-molecule single-cell level. This means that new strains of the virus or other new viruses can be detected easily by designing new probes.

Have you ever wanted to detect very small fragments of genomic DNA but there were no effective tools available? Our novel method based on the CRISPR-Cas9 system and T7 exonuclease, named CRISPR-ExoFISH, now allows to detect genomic loci as small as 2 kb!

Do you have a set of genes whose expression needs to be validated after RNA sequencing or microarray? Do you want to visualize multiple cell types in a tumor microenvironment? NovaFISH is here to help.

NovaFISH is a new kind of single-molecule FISH (smFISH) technique that enables you to quantify gene expression with spatial information and digital quantification. Due to the intrinsic low signal of smFISH (dozens of fluorophores on each stained RNA dot), we have several suggestions on the image acquisition of smFISH samples, including NovaFISH, to get high-quality images for accurate and reliable NovaFISH quantification.