HuluFISH
INDUSTRY'S FIRST TRUE MULTIPLEX SINGLE-MOLECULE FISH WITH NOVEL PROBE DESIGN
HOW HuluFISH WORKS
As the first true multiplexing smFISH method, HuluFISH is our proprietary enzymatic labeling method enabling combinatorial color barcoding on every single probe. This cost-effective enzymatic labeling method combines with our advanced FISH probe design. As a result, your smFISH staining will be brighter and more affordable. The multiplexity power of HuluFISH is revolutionalizing. It is the first time that smFISH probe can have tremendous multiplexity in single hybridization round. And the multiplexity is increasing exponentially with the spectral separable fluorophores.
Chen, Yong-Sheng, et al.
"Autonomous combinatorial color barcoding for multiplexing single molecule RNA visualization."
bioRxiv (2017): 127373.
WHY IS MULTIPLEXITY IMPORTANT
The biological system is never simple. When you want to study gene expression, you will encounter the redundancy and complexity in biology. In fact, you will probably end up with studying a panel of genes involved in the certain pathway. Or when you want to find biomarkers, the accuracy will increase with multiple orthogonal biomarkers. In all these cases, you need an in situ method like HuluFISH to identify all of the genes of interest at one step. This will guarantee you the most faithful expression pattern with spatial information included, and help you identify the mechanisms in your story.
HOW CAN HuluFISH HELP MY RESEARCH
The HuluFISH technology can be applied to any academic or clinical research that requires targeted genetic testing. For example, HuluFISH is a great complementary technology to NGS, providing means for precise, imaging-based gene expression validation. The multiplexity of HuluFISH sheds light on many complex studies such as those in drug discovery, single cell RNA sequencing validation, etc.
Read more about APPLICATIONS.
The HuluFISH smFISH probe is now available as HuluFISH Kit - an all-in-one kit to help you get everything started quickly. Furthermore, we also provide accompanying AI-powered data quantitation service HuluREAD which is completely FREE OF CHARGE. No more manual counting and get insight of your data with just a few clicks.
HuluFISH Kit is fully customizable. Order it now or ask for a quotation!
HuluREAD
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AUTOMATED DATA QUANTITATION
JUST A FEW CLICKS AWAY
> SCIENTIFIC
Designed by scientists.
Developed for scientists.
> FAST AND ACCURATE
Built on state-of-the-art deep learning and image analysis.
> QUANTITATIVE
Abundant imagery features and statistical analysis
> PUBLICATION READY
All you need to know about
your data in a single report.
> SIMPLE
No installation, no tuning.
Calling service in seconds.
> HIGHLY ACCESSIBLE
Fully cloud and web based.
Service accessible from anywhere.
APPLICATIONS
Single Cell Single Molecule Research
We provide highly customizable smFISH probes selected and optimized for your experiments. Furthermore, we provide AI-powered image analysis and data quantitation software completely FREE OF CHARGE.
African Swine Fever Screening
The outbreak of African swine fever (ASF) has cost many hundreds of millions of dollars loss to the Chinese pig farmers in 2018 alone. Together with our business partners, we are developing a universal pathogen detection solution based on HuluFISH to prevent another ASF outbreak.
CAR T-Cell Therapy Quality Control
Together with our clinical parter, our data with CAR T-cell has shown industry’s first successful detection of CAR gene expression in human T cells at single molecule precision with well reproducible batch-to-batch results.
Your Application?
Our technologies live on a platform that is made generic and highly adaptable. We can find expression information of your interest from RNA and DNA at a single cell single molecule level.
Tell us about your application: info@pixelbiotech.com
SINGLE-MOLECULE
PRECISION & MULTIPLEXITY
AT YOUR FINGERTIPS
How to order customized HuluFISH Kit
How to access HuluREAD for free data quantitation
Step 1 – Order online or by email
Order online here or ask for a quotation first.
Describe your target genes (and organisms) using gene names, transcript IDs or FASTA sequences.
We will follow up with details and confirmation.
Step 2 – Receive HuluFISH kit
Receive HuluFISH Kit - a self-contained kit incl.
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HuluFISH Probe(s)
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Accessories
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User Guide
Step 3 – Imaging with HuluFISH
Prepare sample with HuluFISH Kit.
Run imaging experimentation.
Consider using our recommended protocols.
Collect and save your imaging data.
Step 4 – Do data quantitation HuluREAD
Follow the user guide here: HULUREAD USER GUIDE
Upload your imaging data to cloud.
Email link to huluread@pixelbiotech.com.
Relax, enjoy a cup of coffee or read a paper.
Quantitation report will be sent to you automatically.
"We have found the HuluFISH technic highly important for our research, allowing for very high sensitivity,
for detection of various endogenous mRNAs, including low expressed ones. The method is also highly effective
easy "switch"between different tags, allowing for a variety of applications, including co-localization, super-resolution imaging and IP. The artificial intelligence (AI) powered analytics tool for image processing has allowed us to quantify accurately mRNA levels in different cellular compartments."
Dr. Ayala Shiber
Technion - Israel Institute of Technology
"It promises not only a CAR detection requiring very low amount of body liquid (blood, liquor, lymph) but also for the first time ever a molecular quantification
in a single CAR positive cell. This novel method will therefore give us the opportunity to investigate our cellular treatments more specifically and more sensitive. We are hopeful that HuluFISH will become a standard detection method for CARs at the patient bedside."
Dr. Patrick Schmidt
National Center for Tumor Diseases (NCT)
"HuluFISH is an extremely user-friendly single cell molecular FISH technology. The workflow is straightforward even to the researchers, who have no background knowledge of FISH.
The service from PixelBiotech is also wonderful, quick response and troubleshooting. Because of the high sensitivity and absolute quantification
of RNA molecules in each single cell, we use HuluFISH as a complementary approach for the single cell RNA-seq."
Dr. Qin Zhang
German Cancer Research Center (DKFZ)
TESTIMONIALS
PRODUCT AND SERVICE
TRUSTED BY
ABOUT PixelBiotech
PixelBiotech is a German startup and our mission is to deliver cutting-edge technologies for targeted RNA and DNA studies, creating opportunities for scientists to advance research in fields like infectious diseases, cell and gene therapy and drug development.
We offer high-performance and customizable single molecule imaging solutions that answer needs in discovery, translational and clinical research. Our products and services are encapsulated into a unified Hulu platform, integrating molecular biology, chemistry, bioinformatics and artificial intelligence (AI) algorithms. The platform features our proprietary HuluFISH technology - the industry's first true multiplex single molecule FISH (smFISH), and HuluREAD - an AI-powered analytics tool that processes your experimental data.